Table 3.

The expression levels of miR-16-5p and miR-19b-3p normalized to the exogenous miRNA in the total training and validation phases.

	h16c39				h19bc39
	aveΔCt	Sd	2-ave△△Ct	P value	aveΔCt	Sd	2-ave△△Ct	P value
T1	7.811	1.062	0.560	0.001	12.265	1.415	0.601	0.020
T2	8.260	1.134	0.410	<0.001	12.763	1.386	0.425	0.001
T3	8.398	1.002	0.373	<0.001	13.482	1.676	0.258	<0.001
T4	8.500	0.941	0.347	<0.001	13.238	1.056	0.306	<0.001
G1	8.171	0.760	0.436	<0.001	12.650	1.282	0.460	0.011
G2	8.114	1.267	0.454	<0.001	12.785	1.507	0.419	<0.001
G3	8.338	0.980	0.389	<0.001	13.248	1.668	0.304	<0.001
GC	8.217	1.088	0.423	<0.001	12.957	1.589	0.372	<0.001
N	6.975	1.301	1		11.530	1.570	1

aveΔCt, the mean value of the normalized Ct values of the miRNA for each cohort in the specific phase; Sd, the corresponding standard deviation to aveΔCt; 2^-ave△△Ct, the ratio of the average expression level of the miRNA for the special GC cohort to that for the N cohort; T1-4, the GC cohort with different TNM stages respectively from Ⅰ(T1), Ⅱ(T2), Ⅲ(T3) to Ⅳ(T4); G1-3, the GC cohort with different differentiation grades respectively from well(G1), moderately(G2), to poorly(G3) differentiated tumors. h16c39 and h19bc39 denote the normalized Ct value by subtracting the Ct value of cel-miR-39 from that of miR-16-5p and miR-19b-3p. Mann-Whitney U test was used to determine statistical significance at the level of P<0.05.