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. 2015 Mar 12;43(7):3688–3700. doi: 10.1093/nar/gkv152

Figure 3.

Figure 3.

Interactions between mosquito dsRBPs and RNAi/miRNA components. (A) Schematic representation of the Loqs-PA exon structure showing the location of the two Loqs-PA truncations used (Δ258 and Δ226). Start (1) and Stop (329) positions in the ORF are indicated; gray bars above indicate the locations of the three DRMs. The 32 amino acid spacer between DRMs 2 and 3 is highlighted, along with the location of exon 5 when spliced into Loqs-PB. (B) Co-immunoprecipitation of Dcr and Ago proteins with HA-tagged dsRBPs in Aag2 cells. HA-dsRBPs were expressed in Aag2 cells by transfection of plasmid DNA. (C) Co-Immunoprecipitation of FLAG-Loqs-PA or FLAG-Loqs-PB with HA-tagged proteins. Column headings indicate the overexpressed HA-tagged protein, row headings indicate the antibody used in the corresponding western blot. Input (In), flow-through (FT) and bound (B) fractions are indicated. To increase the amount of detectable R2D2, HA-R2D2 proteins were expressed via infection with dsSINV; all others were expressed by plasmid transfection. Anti-HA and anti-FLAG co-IP assays were run 24 h post-infection (48 h post-transfection). (D) Dimerization of Loqs-PA is unaffected by both the Δ258 and Δ226 deletions as shown by co-IP.