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. 2015 Mar 16;43(7):3434–3441. doi: 10.1093/nar/gkv174

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — LC-MS/MS and -MS/MS/MS of the synthetic standard 6 and the enzymatically digested cross-linked duplex B. Panels (a) and (b) are for the synthetic nucleoside 6, while panels (c) and (d) show the cross-link remnant obtained from digestion of duplex B. Panels (a) and (c) are the selected-ion chromatograms for the m/z 368→252 transition. Panels (b) and (d) are the selected-ion chromatograms for the m/z 368→252→136 transition. Shown in the insets are the MS/MS ((a) and (c)) and MS/MS/MS ((b) and (d)) averaged from the 28.4-min fraction in (a) and (b), and 28.8-min fraction in (c) and (d).