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. 2015 Mar 23;43(7):3478–3489. doi: 10.1093/nar/gkv233

Figure 5.

Figure 5.

Sub-network analysis of competing lncACTs. (A) A BRCA-specific sub-network was derived from the global crosstalk network, which consisted of 95 lncRNAs, 68 miRNAs, 197 mRNAs and contained 132 functional modules. Two example modules are demarcated by circles. The lncRNAs, miRNAs and coding-genes are colored blue, red and yellow, respectively. The node degree is indicated by the node size. (B) One module had five nodes, all of which have been experimentally demonstrated to have key roles in the development of breast cancer. The expression heat map is shown on the right. In the heat map, highly expressed genes are shown in red, low expression genes are shown in green. (C) The lncRNA H19 was identified as a hub that connects five competing modules. (D) The lncRNA MIR22HG is functionally complementary to H19 and interacts with the same miRNAs. A genomic representation of miR-22 is shown in the red bar and MIR22HG is shown on the bottom. Predicted miR-22 target sites on MIR22HG are shown in the black bar, with detailed miRNA binding information of the site nearest to the miR-22 locus illustrated on the right. (E) Hierarchical clustering of 248 patients based on H19 and BRCA1/2 lncACT expression. (F) Kaplan-Meier survival analysis of two groups of patients with different clinical outcomes. Those that showed no progression or who were still alive at the time of the last follow-up were censored (+). Survival days are shown along the X axis. Overall survival rates are shown along the Y axis.