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. 2015 Mar 23;43(7):3814–3825. doi: 10.1093/nar/gkv245

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Stable expression of ORRM3 under a 35S promoter in orrm3–1 mutant plants complements the editing defects. orrm3–1, homozygous orrm3–1 mutant plants; orrm3–1 w/ 35S::ORRM3, orrm3–1 mutant plants transformed with a construct expressing ORRM3 driven by a 35S promoter.(A) Several transgenic mutant plants were assayed by PPE assay. E, edited band; U, unedited band; O, oligonucleotide. (B) Editing measured by bulk sequencing shows the complementation to be specific. Portion of electrophoretograms from RT-PCR bulk sequencing is shown. The editable C is shown in white letter in black background. Only the sites being affected in orrm3–1 mutant plants are complemented by stable expression of ORRM3 (bottom lane), whereas the sites not affected in orrm3–1 mutant are not complemented (upper lane).