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. 2015 Mar 23;43(7):3814–3825. doi: 10.1093/nar/gkv245

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Residual extent in orrm3 mutant plants can be reduced by silencing ORRM2. (A) Juxtaposition of editing extent at two sites under the control of ORRM2 and ORRM3 in ORRM2-silenced orrm3 mutant plants and ORRM2 expression. A solid black line withsquares shows the relative ORRM2 expression in orrm3 mutant plants inoculated with Agrobacteria harboring a GFP and ORRM2 co-silencing construct and the controls. Bars refer to the editing extent at sites rps3 C1344 and nad6 leader C-73, measured by PPE assay. NOT, orrm3 mutant plants that were not inoculated with Agrobacteria; GFP-sil, orrm3 mutant plants inoculated with Agrobacteria harboring a GFP silencing construct; sil-1 to sil-8, orrm3 mutant plants inoculated with Agrobacteria harboring a GFP and ORRM2 co-silencing construct. (B) Editing extent in ORRM2-silenced orrm3 mutant plants correlates with the expression of ORRM2.