. 2015 Apr 6;2015:753710. doi: 10.1155/2015/753710

Table 1.

Primers used in PCR for amplification of cagA, vacA, and iceA sequences and s1 region sequencing.

DNA amplified region	Primer	Primer sequence	PCR product	PCR program	Reference
DNA amplified region	Primer	(5′-3′)	(bp)	PCR program	Reference
cagA	CAG-L	TGCTAAATTAGACAACTTGAGCGA	289	30 cycles (1 min at 95°C, 1 min at 50°C, and 1 min at 72°C)	[13]
cagA	CAG-R	AATAATCAACAAACATCACGCCAT		30 cycles (1 min at 95°C, 1 min at 50°C, and 1 min at 72°C)	[13]

vacAs1a	SS1-F	GTCAGCATCACACCGCAAC	190	35 cycles (1 min at 95°C, 1 min at 56°C, and 1 min at 72°C)	[5]
vacAs1b	SS3-F	AGCGCCATACCGCAAGAG	187		[5]
vacAs1c	SS1C-F	CTAGCTTTAGTGGGGATA	213		[14]
vacAs2	SS2-F	GCTAACACGCCAAATGATCC	199		[5]
vacAs2	VA1-R^*	CTGCTTGAATGCGCCAAAC			[5]
vacA m1/m2	VAG-F	CAATCTGTCCAATCAAGCGAG	567/645^±		[5]
vacA m1/m2	VAG-R	GCGTCAAAATAATTCCAAGG	567/645^±		[5]

VacAs1	SIG-F	ATGGAAATACAACAAACACACCG	338		[8]
	SIG-R	CAACCTCCATCAATCTTACTGGA	338		[8]
	VA1-F	ATGGAAATACAACAAACACAC	259		[5]

iceA1	iceA1-F	GTGTTTTTAACCAAAGTATC	246	30 cycles (1 min at 95°C, 1 min at 50°C, and 1 min at 72°C)	[8]
iceA1	iceA1-R	CTATAGCCATTATCTTTGCA	246
iceA2	iceA2-F	GTTGGGTATATCACAATTTAT	229/334^¥
iceA2	iceA2-R	TTTCCCTATTTTCTAGTAGGT	229/334^¥

^*Used as reverse primer with SS1-F, SS3-F, SS1C-F, SS2-F, and VA1-F. ^±The size of the product is variable depending on the present subtype 567 bp for m1 and 645 pb for m2. ^¥The primers yield a fragment of 229 or 334 bp depending on the presence of a repetitive sequence of 105 nucleotides codifying for 35 amino acids in some iceA2 alleles.