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. 2015 Apr 20;5:9538. doi: 10.1038/srep09538

Figure 4. Human Mast Cells are Activated by a Combination of FcεRI-Bound and ‘Free’ SPE-7 IgE.

Figure 4

Percentage degranulation of LAD-2 cells, induced by indicated nM concentration of SPE-7, 102.1F10 and 27–74 IgE antibodies, in non-sensitised cells (A, n = 2–6) and those sensitised with SPE-7 IgE (B, n = 2–5), 27–74 IgE (C, n = 2–3) or 102.1F10 IgE (D, n = 2–6). Statistically significant difference to PBS background control was determined by one-way ANOVA with Dunnett's post-test; *** p < 0.001, ** p = 0.001 to 0.01. Comparison of degranulation in non-sensitised cells or cells pre-sensitised with SPE-7 IgE (E). Statistically significant difference was analysed by one-way ANOVA with Tukey's post-test. All data are shown as mean ± SEM.