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. 2015 May 1;38(5):801–814. doi: 10.5665/sleep.4680

Figure 5.

Figure 5

hll mutants exhibit reduced sleep homeostasis. (A) Levels of hll messenger RNA (mRNA) from tubGS > hllRNAi induced with RU486 are decreased compared to sibling flies uninduced using vehicle control. mRNA is expressed as a percentage of vehicle control (n = 5 flies/group). *P < 0.05 by Student t-test. (B) Sleep homeostasis is reduced when tubGS > hllRNAi are induced with the drug mifepristone (RU486, n = 28) compared to when the flies are treated with the vehicle control (veh, n = 28). (C) mRNA for hll was reduced in whole heads in Actin-Gal4/UAS-hllRNAi flies compared to parental lines (Actin-GAL4/+ and UAS-hllRNAi/+). mRNA levels were normalized to UAS-hllRNAi/+; n = 20 heads/group. *P < 0.05 by planned comparison Student t-test in indicated comparison. (D) Sleep homeostasis is reduced in Actin-Gal4/UAS-hllRNAi (n = 66) compared to Act-GAL4/+ (n = 39) and UAS-hllRNAi/+ (n = 66) parental lines. One-way analysis of variance F2,168 = 7.51; *P < 0.01 modified Bonferroni Test. *P < 0.01 by Student t-test.