Figure 3.
IL-1R−/−, but not IL-1Ra−/−, mice have reduced lung metastases. BALB/c, IL-1R−/−, and IL-1Ra−/− mice were inoculated with 7,000 4T1 tumor cells in the abdominal mammary gland on day 0, and their lungs harvested on days 35 to 40, when BALB/c mice were moribund. Metastatic cells were quantified using the clonogenic assay. Lung metastases are reduced in IL-1R−/− mice relative to BALB/c mice (P < 0.05). ●, number of metastatic cells in the lungs of individual mice. Data are pooled from two to three experiments.