Table 1.
Sense and Antisense Primers Used for Real-Time qPCR (SYBR Green)
| Gene | Accession No. | Sense Primer (5’→3’) | Antisense Primer (5’→3’) |
|---|---|---|---|
| Cyp7a1 | NM_012942.2 | GCAAAACCTCCAATCTGTCAT | GCTTCAAACATCACTCGGTAAC |
| Cyp7b1 | NM_019138.1 | GGTTCTGAGGTTGTGCTCCTAC | GACTTCTGGGTCATTGTGTATCA |
| Cyp8b1 | NM_031241.1 | AGGTTGGAAGCCGAGACAT | TGGCACAGAGACGAAGAGTC |
| Cyp27a1 | NM_178847.2 | CAGCAAGAAGGTGAGCCTAC | TGTCTATGGGAAGGGCAGAG |
| Cyp3a | AB084894.1 | CACAAAGACCCAAAGTGCTG | CCATTACCAAAGGGCAGGT |
| Ugt2b | NM_031533.5 | CCCACCACCGTAGATGAGAC | GCAAGGGTTTAGCAGGTTTG |
| Sult2a1 | NM_131903.1 | AGGAACGAACTGGCTGATTG | ATGGGAAGATGGGAGGTCAT |
| Gsta2 | NM_017013.4 | GCAGGAGTGGAGTTTGATGAG | TTTGGTGGCGATGTAGTTGA |
| Gsta3 | NM_031509.2 | GGCTAAGGAATGATGGGAGTT | TGGTGGCAATGTAGTTGAGAA |
| Gsta4 | NM_001106840.1 | GCTGGAGTGGAGTTTGAAGAA | GATGGCTCTGGTCTGTGTCA |
| Gstm1 | NM_017014.1 | CCCATCTCCTCAACCTCAC | GGGCAGACCTCAAATCACAG |
| Gstm2 | NM_177426.1 | TGCTCCCGACTATGACAGAA | CGTCCACACGAATCCTCTC |
| Gstm3 | NM_020540.1 | TGGACACTTTGGAGAACCAG | CTTGCCCAGGAACTCTGAAT |
| Gstm4 | NM_001024304.1 | TCACACTGGCTCTGGCTTCT | CCCTGCCTATCCAACTGAAAT |
| Mrp2 | NM_012833.1 | CCCAGTCTTCGCTATCATCA | GACAGAATCCAACCGTCTCAG |
| Bsep | NM_031760.1 | CGTGCTTGTGGAAGAAGTTG | GGGAGTAGATGGGTGTGACTG |
| Abcg2 | NM_181381.2 | CTTACTGGCTTCTGGGAAACTC | AGGGTTGTTGTAGGGCTCAC |
| Abcg5 | NM_053754.2 | TAAGATGGCAGGCAGGAAAG | AGCAAAGGACGGTGAGTTCT |
| Abcg8 | NM_130414.2 | CTCATCGTCATTGGCATCA | GTGGAAGCAAGGCTGAACAT |
| Mdr1 | NM_012623.2 | GAGCCCATCCTGTTTGACTG | TGTCTCCCACTCTGGTGTTG |
| Mdr2 | NM_012690.2 | TCAGCAACCAGAGCAGAGAA | GCCCAGGAGCATAAACAAT |
| Mrp3 | NM_080581.1 | TTCCGATTCACCACTTTCTACA | GGCAAGGATTTGTGTCAAGATT |
| Mrp4 | NM_133411.1 | GAAGGAAAATGAGGAAGCAGAG | GGATGACTGTTGAGACCAAATC |
| Ostα | NM_001107087.1 | AAGTCGGAAGGGTTGGGTAG | ATCCTCTGCTGTGCCATCTC |
| Ostβ | XM_001076555.1 | TTTGGTATTTCCGTTCAGAGG | GCATTCCGTTGTCTTGTGG |
| Ntcp | NM_017047.1 | CTGGCTACCTCCTCCCTGAT | ATGCTGATGGTGCGTCTG |
| Oatp1 | NM_017111.1 | TGTATGGAGAACCGAACACAGA | AAGGGCACAATAGGAGTTTCAC |
| Oct1 | NM_012697.1 | TGTGGCTTTGCCTGAGACTA | CTTGCCTGTTTGGACCTGAA |
| Fxr | NM_021745.1 | TGAGCGTCTACAGCGAAAGTG | GGGATGGTGGTCTTCAAATAAG |
| Shp | NM_057133.1 | ATCTCTTCTTCCGCCCTGTC | AGGTTTTGGGAGCCATCAAG |
| Pxr | NM_052980.2 | ACATCATCCCTCACCCTTCA | TCAGGTCTCATCTCCAGGTTTA |
| Car | NM_022941.4 | CCAAGGAACTGTGTGGTGTG | CTGGACAATGGCGTCTCTG |
| Vdr | NM_017058.1 | GCCGCCTGTCTGTGTTATTC | GGTCATCTTGGCAGTGAGTG |
| Pparα | NM_013196.1 | CGGTGTGTATGAAGCCATCTT | TCTTTAGGAACTCTCGGGTGAT |
| Hnf1α | NM_012669.1 | AGAGGGAAGCAGGGTGAAG | CACAGAAATCCAGGCAGTCA |
| Hnf4α | NM_022180.2 | TAGCAGAGATGAGCCGTGTG | GCTTTGAGGCAGGCGTATT |
| Rxrα | NM_012805.2 | TTCTCCCACCGCTCCATAG | CGTTAGCACCCTGTCAAAGA |
| Rarα | NM_031528.2 | CACCTGAGCAAGACACAATGA | GCGAAGGCAAAGACCAAGT |
| Lxr | NM_031626.1 | CGCTACAACCACGAGACAGA | GGCAATGAGCAAGGCATACT |
| Lrh-1 | NM_021742.1 | ATGGGAAGGAAGGGACAATC | CAAACTGAAGGGAACGGAGTC |
| Nrf2 | NM_031789.2 | CCTTCCTCTGCTGCCATTAG | GTGCCTTCAGTGTGCTTCTG |
| Hnf3β | NM_012743.1 | AACAAGATGCTGACGCTGAG | GAATGACGGATGGAGTTCTG |
| Ahr | NM_013149.2 | CAGGACCAGTGTAGAGCACAAG | CTGCCGTGACAACCAGAAC |