Western blot assays for PI3K, Akt and eNOS. Indicated representative western blotting analysis of PI3K, Akt and eNOS protein levels (A), statistical analysis of PI3K, Akt and eNOS protein levels (B-D), respectively, in different groups. **P < 0.01 compared with Con group; ##P < 0.01 compared with IRI group. Con, control group; IRI, ischemia/reperfusion-injury group; Cat (25), catalpol (25 mg/kg)-treated group; Cat (50), catalpol (50 mg/kg)-treated group; Cat (100), catalpol (100 mg/kg)-treated group.