Figure 2.

miR-210 mimic induced EMT in SKOV3 ovarian cancer cells. A. miR-210 level in SKOV3 cells transfected with miR-210 mimic or negative control, respectively, was analyzed using real-time RT-PCR assay. Compared with negative control cells, miR-210 level was significantly increased in cells transfected with miR-210 mimic. B. mRNA level of E-cadherin and vimentin in cells transfected with miR-210 mimic or negative control, respectively, was monitored by real-time RT-PCR. Compared with negative control cells, E-cadherin decreased and vimentin increased in cells transfected with miR-210 mimic. C. E-cadherin and vimentin protein levels in cells transfected with miR-210 mimic or negative control were examined by western blot. E-cadherin protein was decreased and vimentin protein was increased when miR-210 was overexpressed. D. Cell mobility was detected by wound healing assay. miR-210 mimic promoted the closure of the scratch. The photographs were captured using a phase contrast microscopy (100 ×). All of the treatments in this figure were carried out in triplicate, and the results were displayed as the means ± SD. *P < 0.05, **P < 0.01, t-test.