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Detection of GLUT-1 in MKN45 cells following GLUT-1 shRNA transfection. GLUT-1 mRNA and protein levels in cells detected using western blot analysis (A) and RT-PCR (B), respectively. (C) Detection of GLUT-1 in transfected and untransfected MKN45 cells by immunofluorescence. Nuclei were stained with DAPI. Untransfected: Untransfected MKN45 cells; Mock: MKN45 cells transfected with control shRNA Plasmid-A; Knockdown: MKN45 cells transfected with GLUT-1 shRNA.
