Table 1.
Genes | Primer Sequences (5’ → 3’) | Expected size of the PCR product (bp) | Restriction enzymes | Annealing temperature (°C) |
---|---|---|---|---|
RAD51 | F: GGGAACTGCAACTCATCTGG | 157 | MvaI | 62.5 |
R: GCGCTCCTCTCTCCAGCAG | ||||
XRCC2 | F: TGTAGTCACCCATCTCTCTGC | 290 | HphI | 61 |
R: AGTTGCTGCCATGCCTTACA |
F: Forward, R: Reverse.