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. 2015 Feb 15;8(2):2649–2655.

Table 1.

RAD51 and XRCC2 gene polymorphisms, primers, PCR product sizes, restriction enzymes, and annealing temperatures

Genes Primer Sequences (5’ → 3’) Expected size of the PCR product (bp) Restriction enzymes Annealing temperature (°C)
RAD51 F: GGGAACTGCAACTCATCTGG 157 MvaI 62.5
R: GCGCTCCTCTCTCCAGCAG
XRCC2 F: TGTAGTCACCCATCTCTCTGC 290 HphI 61
R: AGTTGCTGCCATGCCTTACA

F: Forward, R: Reverse.