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. 2015 Feb 15;8(2):3063–3071.

Table 1.

Oligonucleotide sequences used for real-time PCR

Gene Sequences (5’-3’) Hybridization T°C Cycles
ABCB1 Forward: AAAAAGATCAACTCGTACCACTC 65 37
Reverse: GCACAAAATACACCAACAA
ABCC2 Forward: CTGGGAACATGATTAGGAAGC 65 42
Reverse: GAGGATTTCCCAGAGCCGAC
ABCG2 Forward: GGGTTCTCTTCTTCCTGACGACC 65 38
Reverse: CCAGACAACCAGTTAGAGTGTTGGT
Survivin Forward: GCATGGGTGCCCCGACGTTG 60 35
Reverse: GCT CCG GCC AGA GGC CTC AA
BCL-2 Forward: ACGGGGTGAACTGGGGGAGGA 60 35
Reverse: TGTTTGGGGCAGGCATGTTGACTT
HIF-1 Forward: TGCTCATCAGTTGCCACTTCC 60 45
Reverse: CGCTGTGTGTTTTGTTCTTTACCC
TGF-β Forward: TGTGTGACGAGCCCAAGGA 65 45
Reverse: TAGTTGGGTCTGGGCCAAAC
CDK1 Forward: CAG TCT TCA GGA TGT GCT TAT GC 65 45
Reverse: GAGGTTTTAAGTCTCTGTGAAGAA CTC
GST Forward: ACGTGGCAGGAGGGCTCACTC 60 40
Reverse: TACTCAGGGGAGGCCAGCAA
β-actin Forward: TGAGCGCGGCTACAGCTT 60 40
Reverse: TCCTTAATGTCACGCACGATTT