Table 1.
Oligonucleotide sequences used for real-time PCR
Gene | Sequences (5’-3’) | Hybridization T°C | Cycles |
---|---|---|---|
ABCB1 | Forward: AAAAAGATCAACTCGTACCACTC | 65 | 37 |
Reverse: GCACAAAATACACCAACAA | |||
ABCC2 | Forward: CTGGGAACATGATTAGGAAGC | 65 | 42 |
Reverse: GAGGATTTCCCAGAGCCGAC | |||
ABCG2 | Forward: GGGTTCTCTTCTTCCTGACGACC | 65 | 38 |
Reverse: CCAGACAACCAGTTAGAGTGTTGGT | |||
Survivin | Forward: GCATGGGTGCCCCGACGTTG | 60 | 35 |
Reverse: GCT CCG GCC AGA GGC CTC AA | |||
BCL-2 | Forward: ACGGGGTGAACTGGGGGAGGA | 60 | 35 |
Reverse: TGTTTGGGGCAGGCATGTTGACTT | |||
HIF-1 | Forward: TGCTCATCAGTTGCCACTTCC | 60 | 45 |
Reverse: CGCTGTGTGTTTTGTTCTTTACCC | |||
TGF-β | Forward: TGTGTGACGAGCCCAAGGA | 65 | 45 |
Reverse: TAGTTGGGTCTGGGCCAAAC | |||
CDK1 | Forward: CAG TCT TCA GGA TGT GCT TAT GC | 65 | 45 |
Reverse: GAGGTTTTAAGTCTCTGTGAAGAA CTC | |||
GST | Forward: ACGTGGCAGGAGGGCTCACTC | 60 | 40 |
Reverse: TACTCAGGGGAGGCCAGCAA | |||
β-actin | Forward: TGAGCGCGGCTACAGCTT | 60 | 40 |
Reverse: TCCTTAATGTCACGCACGATTT |