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DxP consensus sequence. Fig. 2A showed the consensus sequence between DSPP and human Chordin C-terminal cleavage sites. As shown in Fig. 2C, a number of between mutations were generated in the P2′ position of DSP-PP. Samples were run on PAGE and stained with Stains-All. The D449G (Fig. 2B) mutation resulted in a PP240 band 1/3 normal. Gel data from the other substitutions are derived from Yang, et al., 2013 (2).
