. 2015 Jan 28;79(1):39–60. doi: 10.1128/MMBR.00031-14

TABLE 2.

Kinetic parameters of DCS, CURS1, CURS2, and CURS3 from C. longa

EC no.	Enzyme^a	GenBank accession no.	Substrate	K_m (μM)	k_cat (s⁻¹)	k_cat/K_m (s⁻¹ M⁻¹)	Reference
2.3.1.218	DCS	AB495006	Feruloyl-CoA	–	0.020	–	97 ^b
2.3.1.218	DCS	AB495006	Malonyl-CoA	8.4	0.011	1,329
2.3.1.217/2.3.1.219	CURS1	AB495007	Feruloyl-CoA	18	0.018	1,001	97
2.3.1.217/2.3.1.219	CURS1	AB495007	Coumaroyl-CoA	189	0.014	75
	CURS2	AB506762	Feruloyl-CoA	4.3	0.007	1,622	102
	CURS2	AB506762	Coumaroyl-CoA	89	0.016	176
	CURS3	AB506763	Feruloyl-CoA	2.2	0.003	2,017	102
	CURS3	AB506763	Coumaroyl-CoA	3.4	0.006	1,742

DCS, diketide-CoA synthase; CURS, curcumin synthase/demethoxycurcumin synthase.

Kinetic properties of DCS for feruloyl-CoA were calculated according to its allosteric properties (sigmoidal curve). The S₅₀, k_cat, and Hill slope values were 46 μM, 0.02 s⁻¹, and 1.8, respectively.