TABLE 6.
Comparison of kinetic parameters of 4-coumarate-CoA ligases (EC 6.2.1.12) from different organismsa
| Organism | Enzyme | Substrate | Km (μM) | Sp act (nmol s−1 mg−1) | Sp act/Km (μl s−1 mg−1) |
|---|---|---|---|---|---|
| Petroselinum crispum | Pc4CL2 | p-Coumaric acid | 11.8 | 1.983 | 168.051 |
| Caffeic acid | 8.4 | 1.533 | 182.540 | ||
| Ferulic acid | 11.2 | 1.367 | 122.054 | ||
| Arabidopsis thaliana | At4CL1 | p-Coumaric acid | 28.7 | 11.550 | 403.439 |
| Caffeic acid | 15.7 | 5.267 | 335.478 | ||
| Ferulic acid | 40.8 | 6.800 | 166.667 | ||
| At4CL2 | p-Coumaric acid | 45.1 | 10.433 | 231.330 | |
| Caffeic acid | 7.9 | 8.483 | 1,073.797 | ||
| Ferulic acid | ND |
a
Data are from reference 175. ND, not determined due to very low activity.