Skip to main content
. 2015 Spring;14(2):617–626.

Figure 1.

Figure 1

OE-PCR protocol for the insertion of the OmpA signal sequence into the TRAIL cDNA. Reaction 1: hybridization of 3´ region of primers F1 with terminal regions of TRAIL cDNA and synthesis a part of the OmpA signal sequence. Reaction 2: using the product of the reaction 1 as a template by primer F2 and completion of whole OmpA signal sequence.