Skip to main content
. 2015 Mar 30;112(15):E1936–E1945. doi: 10.1073/pnas.1421480112

Fig. 3.

Fig. 3.

Loss of SOX2 in adult hipNPCs reduces the expression of NGN2 and NEUROD1 in early neuroblasts. (A) Graphical representation of the expression of NGN2, NEUROD1, and PROX1 in adult hippocampal neuronal maturation. (B, Left) Sections from 2-mo-old mice were immunostained for NGN2. (Scale bar: 20 µm.) (Right) Quantification results are expressed as the average intensity of NGN2 staining in stem/progenitor (CFPnuc+) cells relative to mature granule neurons. (C, Left) Sections from 2-mo-old mice were immunostained for NEUROD1. (Scale bar: 50 µm.) (Right) Quantification results are expressed as the average intensity of NEUROD1 staining in neuroblasts (DCX+) cells relative to mature granule neurons. (D, Left) Confocal imaging showing colocalization of NEUROD1 in neuroblasts (DCX+) of wild-type and SOX2cKO mice. (Scale bar: 20 µm.) (Right) Quantification results are expressed as the average intensity of NEUROD1 staining in NPCs, early, mid, and late neuroblasts (NB) and neurons (NeuN). For all quantifications, data are plotted as the mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001).