Fig. 3.

Growth curves of viruses in different cells. (A) Insect C6/36 cells or (B) mammalian LLC-MK2 cells were infected with virus variants at a multiplicity of infection (MOI) of 0.001. Virus titer was measured by plaque assay on BHK cells. (C) NS5^hmin growth kinetics in different cells. C6/36 or LLC-MK2 cells were infected with NS5^hmin at a MOI of 1. Virus titer was measured by plaque assay on BHK cells. (D) Virus titers, measured by focus forming assays in C6/36, BHK-21, Vero E6, A549, or LLC-MK2 cell lines. (E) Effect of Jak inhibitor 1 treatment on virus titer. LLC-MK2 cells were pretreated with Jak inhibitor 1, and the fold change in virus titer relative to untreated cells was measured by a 50% tissue culture infectious dose (TCID₅₀) assay at three and seven days postinfection. Significant differences from D2-syn in D and E are marked by *P value < 0.05 by Wilcox rank sum test.