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. 2015 Mar 30;112(15):4618–4623. doi: 10.1073/pnas.1422088112

Fig. 1.

Fig. 1.

Loss of imprinting in LOS. (A) Flowchart of methodology. (B) Allele-specific expression analysis of imprinted genes identified by RNAseq in day ∼105 B. t. indicus x B. t. taurus fetal kidney. The parental origin of imprinted gene expression (IGE) is defined according to the known imprinting status in human and/or mouse. Samples are arranged from left to right in ascending order by weight and by treatment group. A sample with at least 15% expression from the repressed allele was considered biallelically expressed. Genes that showed biallelic expression in LOS but not in control fetuses are indicated by red boxes. Missing data for RTL1 indicate lack of discriminating SNPs between parental alleles in the reads. We obtained the allelic expression of the long ncRNA KCNQ1OT1 (indicated by asterisk) in a previous study (29). (C) Heat map illustrating the degree of loss of imprinting for the biallelically expressed genes. Numbers represent the percentage of transcripts expressed from the normally repressed allele. A “0” represents <15% expression from the repressed allele (actual percentages may be found in SI Appendix, Table S3).