Table 2.
Binding of oligomers to IgG1-κ and TNFα
| IgG1-κ binding ELISA | TNFα–TNFR1 inhibition ELISA | ||
| Oligomer | IC50, μM* | Oligomer | IC50, μM† |
| α/β-IgG-1 | 3.3 | Z-TNFα | 0.0048 |
| α/β-IgG-2 | 1.4 | α/β-TNFα-1 | 0.030 |
| α/β-VEGF-1 | >60 | α/β-TNFα-2 | 0.0066 |
| α/β-TNFα-1 | >60 | α/β-VEGF-1 | >10 |
| α/β-IgG-1 | >10 | ||
*
IC50 values are based on the ability of each oligomer to compete with immobilized α/β-IgG-2 for binding to 10 μg/mL human IgG1-κ.
†
IC50 values are based on the ability of each oligomer to compete with immobilized TNFR1 for binding to 0.081 μg/mL TNFα.