Fig. 1.

Design of protease cleavable MDMXc3. (A) Identification of MDMX disordered regions for the insertion of cleavage sites. PreScission cleavage site and epitope tags were inserted after residues 140, 350, and 429 to create MDMXc3. Cleavage by PreScission protease produces four fragments each containing a unique epitope. (B) Lysate of H1299 transfected with MDMXc3 was digested with 0.1 µg/µL PreScission for 10 min at 4 °C and analyzed by Western blot to detect the production of individual fragments.