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. 2015 Mar 30;112(15):E1871–E1879. doi: 10.1073/pnas.1423204112

Fig. 1.

Fig. 1.

Effect of JH treatment on the intracellular levels of calcium, cAMP, DAG, and IP3 in mosquito Aag2 cells. Cells were treated with the indicated chemicals (1 µM) or ethanol (0.1%) as a solvent control. (A) Cytoplasmic calcium concentration was measured using the Fluo-8 AM fluorescent calcium indicator and a microplate reader. Values are expressed as relative fluorescence intensity. (B) Intracellular cAMP contents were assessed after the JH treatment. The adenylate cyclase activator forskolin was used as a positive control. (C and D) The amounts of DAG (C) and IP3 (D) were determined in cells 5, 10, 30, 45, and 60 min after the addition of JH-III. All experiments were repeated three times with similar results. Data are reported as the mean and SD of triplicate samples from a representative experiment.