Fig. 2.

JH treatment causes a rise in intracellular levels of calcium, DAG, and IP₃ in mosquito tissues. (A) JH-stimulated calcium responses in the fat body, Malpighian tubules, midgut, and ovaries. Tissues dissected from adult female mosquitoes within 30 min PE were preincubated with Fluo-8 AM in APS for 1 h, followed by incubation with 1 μM JH-III or ethanol for 15 min. Images were captured using a Zeiss LSM 510 confocal microscope at 1,000× magnification. Cell nuclei were stained blue with DAPI, whereas the binding of calcium to Fluo-8 AM greatly enhanced the green fluorescence intensity. Representative images were taken from one of three independent experiments with similar results. (B) JH treatment increased the production of DAG and IP₃ in cultured fat bodies that were isolated from newly emerged mosquitoes. Fat bodies were incubated in the tissue culture medium with JH-III at a final concentration of 1 µM. Ethanol was used as a negative control. The experiment was repeated twice with three replicates per treatment.