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JH treatment leads to activation of CaMKII. Fat bodies from newly emerged adult female mosquitoes were cultured in vitro for 1 h in the presence of JH-III (1 µM), U73122 (1 µM), BAPTA-AM (10 µM), and/or KN93 (10 µM). Proteins then were extracted from the fat bodies and subjected to Western blot analysis using antibodies against CaMKII phosphorylated at threonine 286 (p-CaMKII; Cell Signaling Technology) and total CaMKII (Cosmo Bio USA).
