Fig. 6.

Blocking the PLC/Ca²⁺/CaMKII pathway reduces the MET/TAI-mediated gene expression. (A) Aag2 cells were transfected with expression plasmids for AaMET and AaTAI, together with a 4×JHRE-luc firefly luciferase reporter construct and a constitutively expressing Renilla luciferase construct. Transfected cells were preincubated with the indicated inhibitors for 1 h followed by treatment with 1 µM JH-III for 4 h. Results are expressed as the ratio of firefly to Renilla luciferase activity and are the mean ± SD of at least three independent experiments. (B) Aag2 cells were transfected with an expression vector for GAL4, together with a 4×UAS-luc firefly luciferase reporter construct and a constitutively expressing Renilla luciferase construct. Transfected cells were treated with the indicated inhibitors and JH-III as described in A.