Fig. 8.

JH treatment induces phosphorylation of AaMET and AaTAI in mosquitoes. Abdomens from newly emerged mosquitoes were cultured in vitro with ethanol or 1 µM JH-III for 2 h. To inactivate CaMKII, the tissues were preincubated with 10 µM KN93 for 1 h before JH-III was added to the culture. Cell extracts were prepared and analyzed by 2D gel electrophoresis followed by Western blot analysis as described in Materials and Methods. A portion of the protein extracts from the JH-treated sample was incubated with λPP (2.5 U/μL) before IEF and SDS/PAGE. AaMET (A) and AaTAI (B) were visualized by immunoblotting with specific antibodies against AaMET and AaTAI.