FIG. 7.

DA-CM-mitigated UVB decreased cell proliferation in HDFs. HDFs were cultured in DA-CM and exposed to sham or UVB irradiation. The cells were stained by EdU and Hoechst 2 days post 5-day UVB irradiation (A). The percentage of EdU-positive cells was shown (B). CCK-8 assay was performed and the absorbance at 450 nm was determined using a Varioskan Flash to evaluate the proliferation ability of four groups of cells (C). Data are mean±SD, n=3, *P<0.05 for SIPS-HDF versus HDF and SIPS-HDF+DA-CM versus SIPS-HDF. EdU, 5-ethynyl-2′-deoxyuridine; HDFs, human dermal fibroblasts; SIPS, stress-induced premature senescence; UVB, ultraviolet B. Color images available online at www.liebertpub.com/scd