Figure 2.
HPK1 expression in HPDE cells and human PDA cell lines. A, 150 µg of cell lysate from each cell line were resolved by 10% SDS-PAGE and immunoblotted with an anti-HPK1 antibody or anti-actin as loading control. B, RT-PCR results showing HPK1 mRNA expression in the HPDE cells and eight different human PDA cell lines.Panc-02, a mouse PDA cell line, was used as the specificity control. C, QRT-PCR results showing HPK1 mRNA expression levels in eight PDA cell lines relative to those in the HPDE cells. D, QRT-PCR results showing HPK1 mRNA expression levels in the ductal epithelial cells in normal pancreas (Normal), chronic pancreatitis (CP), and PDA.