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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Protein Expr Purif. 2015 Feb 14;110:89–94. doi: 10.1016/j.pep.2015.02.007

Fig. 3.

Fig. 3

Optimization of TEV protease cleavage reaction. Two-fold serially diluted TEV protease was added to the histone octamer purified from the metal-affinity chromatography. Cleavage reaction was carried out at 4 °C for overnight. M; size markers (kDa).