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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Protein Expr Purif. 2015 Feb 14;110:89–94. doi: 10.1016/j.pep.2015.02.007

Fig. 4.

Fig. 4

Reconstituted nucleosome from the purified histone octamer serves as a valid substrate to histone methyltransferases. (a) 4-12% native PAGE of the reconstituted nucleosome. (b) DOT1L HMTase assay demonstrating inhibition of DOT1L by SAH with recombinant and extracted nucleosomes. (c) Human MLL1 HMT activity assay using reconstituted nucleosomes exhibits similar methyltransferase activity to HeLa cell extracted nucleosomes. Each data was performed in duplicate.