Fig. 4.

siRNA silencing of p65/rel A was highly effective in vitro. A, to examine the effectiveness of p65/relA silencing on NFκB activity in the cancer cells, PANC-1 cells expressing NFκB luciferase gene were transfected with control siRNA and relA siRNA. After 72 h, cells were treated with or without TNF-α (10 ng/mL) and bioluminescent imaging was used to estimate NFκB reporter activity. B, Western blotting was conducted 72 h after treatment with control siRNA or siRNA against p65/relA in the six cell lines. Full-length gels are presented in Supplementary Fig. S1. Columns, mean for three experiments; bars, SE. *, P < 0.05, versus control.