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. Author manuscript; available in PMC: 2015 Apr 20.
Published in final edited form as: Biochim Biophys Acta. 2013 Sep 30;1838(1 0 0):185–192. doi: 10.1016/j.bbamem.2013.09.014

Fig. 2.

Fig. 2

Binding site of KAHRP in ankyrin R. (A) Schematic representation of ankyrin R functional domains. (B) Binding of K1D to functional domains of ankyrin R. The binding of GST-tagged K1D fragment to MBP-tagged ankyrin R domains was assessed by MBP pull-down assay (left panel) and ELISA (right). Note that K1D bound to only the MBD of ankyrin R. (C) Binding of K1D fragment to subdomains of the MBD domain of ankyrin R. The binding of GST-tagged K1D fragment to sub-domains of MBD was assessed by MBP pull-down assay (a) and by ELISA assay (b). Note that K1D bound to only the D3 region of the MBD domain of ankyrin R.