Skip to main content
. Author manuscript; available in PMC: 2015 Apr 20.
Published in final edited form as: Biochim Biophys Acta. 2013 Sep 30;1838(1 0 0):185–192. doi: 10.1016/j.bbamem.2013.09.014

Fig. 3.

Fig. 3

Kinetic analysis of interaction between K1D and ankyrin R polypeptides by surface plasmon resonance assay. A: GST-tagged K1D fragment was immobilized onto a CM5 sensor chip. MBP, MBP-MBD or MBP-D3 at a concentration of 1 μM was injected at 20 μl/min over the chip surface in a BIAcore 3000 instrument. B: MBP-MBD at different concentrations as indicated was injected at 20 μl/min over the surface in a BIAcore 3000 instrument. The figure shows dose–response curves of MBP-MBD binding to K1D. C: MBP-D3 at different concentrations as indicated was injected at 20 μl/min over the surface in a BIAcore 3000 instrument. The figure shows dose–response curves of MBP-D3 binding to K1D.