Figure 5.

GID1s control female fertility and pod elongation.

(a) Maternal effect of the gid1 null mutations. Flowers of gid1 mutants and wild-type (WT) were emasculated 1 day before anthesis and hand pollinated at anthesis with WT pollen. Mature fruits were individually harvested, and fruit length and seed number were measured. Ratio (seed number versus length) was determined. Values were normalized to the WT.

(b) Gibberellin (GA) response of unfertilized pistils of the gid1 null mutants as measured by relative pistil length in comparison to untreated WT pistils. Flowers of GID1 mutants and WT were emasculated 1 day before anthesis; half of the pistils were treated at anthesis with 300 μm GA₃ (+GA₃), and the other half were treated with mock solution (−GA₃). Fruit or pistil length was measured at 10 dpa. Mean and standard deviation (SD) were calculated from at least 50 pistils/fruits per treatment. The experiment was repeated three times with similar results. Significant differences (Student’s t-test analysis) between the WT and mutants are marked with asterisks (**P-value < 0.001).