Skip to main content
. 2015 Apr 15;29(8):791–802. doi: 10.1101/gad.259341.115

Figure 3.

Figure 3.

IPT is dependent on actin polymerization. NK cells were cocultured with growing, DIS, or OIS cells. NK cells were then collected, stained for CD56 and with DAPI, and analyzed by FACS for the presence of mCherry. (A) The percentage of T+ NK92 cells following 2 h of coculture in the presence of 200 nM LatA or vehicle (DMSO). (B) The percentage of T+ primary human NK cells following 1 h of coculture in the presence of 200 nM LatA or vehicle. (C) CDC42, RAC1, RAC2, and RHOA siRNAs induce efficient knockdown 3 d after transfection. (D) DIS cells with knockdown of CDC42, RHOA, or RAC1 and RAC2 were cocultured for 2 h with NK92 cells. (E) DIS cells were treated with ML141, CASIN (CDC42 inhibitors), or DMSO (control); washed; and then cocultured with NK92 cells. (F) Western blot analysis of CDC42 levels in growing, DIS, and OIS IMR-90 cells. (G) NK-mediated cytotoxicity toward DIS cells following CDC42 knockdown (siCDC42). Results are expressed as means ± SEM from at least three independent experiments. (***) P < 0.001.