Figure 4.

The effect of G_i- α₂AR inhibition, increased cAMP or β₃AR signaling on the plasma concentration of norepinephrine and epinephrine. Total G_i inhibition was achieved by PTX and α₂AR inhibition by L-659,066. Increased cAMP signaling was done by preventing its degradation using the PDE3 inhibitor milrinone. BRL37344 was used to stimulate β₃AR signaling. Significant differences between the control groups pre-treated with PBS and the experimental groups (^* above columns), and between corresponding groups pre-treated with milrinone or β₃AR agonist without and with additional pre-treatment with L-659,066 (^* in bar) were located as indicated. ^* P ≤ 0.05.