Table 2.
Identifications by mass spectrometry of proteins undergoing significant redox remodeling in the sternohyoid muscle after 6 weeks of sustained hypoxia.
| Spot # | Protein | Mw (Da) | GI number | Mascot score | Matched peptides | Sequence coverage | Carbonyl p-value, fold | Free thiol p-value, fold | Expression p-value, fold |
|---|---|---|---|---|---|---|---|---|---|
| ELECTRON TRANSPORT CHAIN | |||||||||
| 1 | Cytochrome bc - 1 complex subunit 1 | 52,819 | 341941780 | 430 | 6 | 26% | - | <0.01, −1.4 | <0.001, −2 |
| 2 | ATP Synthase subunit α | 59,716 | 416677 | 997 | 6 | 53% | - | - | <0.01, +1.5 |
| TCA CYCLE | |||||||||
| 3 | Aconitase hydratase | 85,410 | 60391212 | 1210 | 11 | 41% | <0.01, +1.4 | <0.001, −2 | <0.01, −2.6 |
| 4 | 2-oxo-glutarate dehydrogenase, mt | 116,375 | 146345472 | 477 | 4 | 22% | - | <0.01, −2.4 | <0.01, +2.8 |
| GLYCOLYSIS | |||||||||
| 5 | Fructose bis-phosphate aldolase A | 39,331 | 113607 | 588 | 3 | 70% | - | <0.01, −2.9 | - |
| 6 | Glyceraldehyde-3-phosphate dehydrogenase | 35,787 | 120702 | 531 | 4 | 48% | - | <0.01, −1.6 | <0.001, −2 |
| 7 | Phosphoglycerate kinase 1 | 44,534 | 1730519 | 534 | 4 | 45% | - | - | <0.01, +1.7 |
| 8 | Phosphoglycerate mutase 2 | 28,809 | 6093745 | 1010 | 9 | 57% | - | - | <0.01, +1.7 |
| 9 | Pyruvate kinase isozymes M1/M2 | 57,305 | 146345448 | 837 | 6 | 53% | <0.01, −1.2 | - | <0.01, +1.3 |
| PHOSPHAGEN AND LIPID METABOLISM | |||||||||
| 10 | Glycerol-3-phosphate dehydrogenase [NAD(+)] | 37,548 | 121557 | 412 | 3 | 48% | - | <0.01, −2 | <0.01, −2.6 |
| 11 | Creatine kinase m-type | 43,014 | 124056470 | 784 | 7 | 42% | <0.01, +1.2 | - | <0.01, −1.2 |
| STRESS RESPONSE AND IRON HOMEOSTASIS | |||||||||
| 12 | Glycogen phosphorylase | 97,225 | 14916635 | 1450 | 13 | 47% | <0.01, −1.3 | - | <0.01, +4.1 |
| 13 | 90kDa heat shock protein | 83,229 | 341941065 | 742 | 6 | 31% | - | - | <0.01, −1.3 |
| 14 | Alpha crystallin B chain | 20,056 | 6166129 | 761 | 7 | 65% | <0.01, +1.2 | - | - |
| 15 | Myoglobin | 17,059 | 127676 | 923 | 7 | 43% | - | - | <0.01, −1.7 |
| 16 | Serotransferrin | 76,674 | 21363012 | 121 | 1 | 17% | <0.01, −1.4 | <0.01, +1.8 | - |
| 17 | Carbonic anhydrase III | 29,348 | 30581036 | 966 | 8 | 71% | <0.01, +1.2 | <0.01, −2.7 | <0.01, +1.9 |
| 18 | Albumin | 68,648 | 5915682 | 1340 | 11 | 53% | - | - | <0.01, −1.8 |
| CROSS-BRIDGE AND SARCOPLASMIC RETICULUM | |||||||||
| 19 | Calsequestrin | 46,420 | 341940315 | 543 | 4 | 19% | <0.01, −1.5 | - | <0.01, −2.3 |