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. 2015 Apr 10;6:6789. doi: 10.1038/ncomms7789

Figure 10. Let-7a is required for spine plasticity associated with LTP.

Figure 10

(a) Cultured hippocampal slices were transduced with lentivirus-expressing let-7a sponge and stimulated with high-frequency stimulation for LTP induction; n=5 slices. The fEPSP slope normalized to the baseline prior to stimulation is plotted as mean±s.e.m. In b–d, cultured hippocampal neurons were co-transfected with the let-7a sponge and the venus (for visualization of transfected neurons) construct at DIV14 and stimulated with TEA (25 mM, 15 min) at DIV17. (b) Representative images. (c,d) Quantification of b; n=12–18 neurons for each condition; data are presented as mean±s.e.m. Kruskal–Wallis and Mann–Whitney U-tests are used for statistical analysis. *** and ###P<0.001, **P<0.01. Scale bar, 20 μm for low-magnification images and 5 μm for high-magnification images.