TABLE 1.
Frequency of nonsynonymous, consensus mutations within passaged viral lineages
| Mutation | Amino acid change | Frequency (% of population) |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Passage 0 | Passage 16 |
|||||||||||||
| D1 | D2 | D3 | R1 | R2 | R3 | A1 | A2 | A3 | F1 | F2 | F3 | |||
| HA G483T | A151S | 3.6 | 0.4 | 0.7 | —a | 47.3 | 81.4 | 54.9 | 3.3 | 0.8 | 5.2 | 3.7 | 6.2 | 2.3 |
| HA T627C | S199P | 11.5 | 1.9 | 1.1 | 2.2 | 50.1 | 81.6 | 53.1 | 2.9 | 0.6 | 5.1 | 3.6 | 5.6 | 2.6 |
| HA G634A | S201N | — | — | — | — | 1.2 | 1.2 | 53.0 | — | 0.1 | — | 0.7 | 0.7 | 0.7 |
| HA A641C | E203D | — | 96.5 | 96.8 | 96.5 | — | 0.8 | — | 87.4 | 94.7 | 86.4 | 93.0 | 92.2 | 95.0 |
| HA A1089G | I353V | 6.7 | 0.7 | 0.8 | 0.6 | 47.1 | 81.7 | 55.7 | 3.0 | 0.5 | 5.4 | 4.1 | 6.5 | 4.5 |
| HA A1404G | M458V | 24.0 | 1.8 | 0.9 | 2.4 | 62.9 | 84.4 | 59.9 | 7.5 | 0.4 | 7.7 | 3.6 | 5.6 | 2.7 |
| M A792G | T27A M2 | 5.8 | 37.0 | 38.3 | 38.1 | 56.7 | 29.3 | 8.1 | 66.3 | 74.0 | 39.7 | 58.1 | 62.4 | 55.3 |
| M G434A | A137T M1 | 0.5 | 24.5 | 31.3 | 28.1 | 8.9 | 30.2 | 45.8 | 21.3 | 15.7 | 53.1 | 15.6 | 14.1 | 7.8 |
| NA G412A | S131N | 4.6 | 4.9 | 7.0 | 7.6 | 7.1 | 10.6 | 29.9 | 60.5 | 46.7 | 46.0 | 11.5 | 14.8 | 22.0 |
| NP T906A | S287R | — | — | — | — | — | — | — | 2.2 | 63.7 | — | 0.5 | — | — |
| NS A575G | E26G NS2 | 35.3 | 33.8 | 30.2 | 25.5 | 17.0 | 36.5 | 31.9 | 64.5 | 55.4 | 57.6 | 61.5 | 71.2 | 63.3 |
| PA G292A | V90I | 5.9 | 60.6 | 64.8 | 65.9 | 4.9 | 31.9 | 27.6 | 51.9 | 47.5 | 46.8 | 67.7 | 81.1 | 80.9 |
| PA C314T | T97I | — | — | — | — | 88.3 | 10.9 | 55.2 | — | — | — | 0.7 | 0.9 | — |
| PB1 G1959A | M645I | — | — | — | — | 90.3 | 44.6 | 14.5 | — | — | — | 0.3 | — | — |
| PB2 G2128A | D701N | — | 45.6 | 64.5 | 63.1 | 1.5 | 1.9 | 3.2 | 19.4 | — | 20.8 | 65.8 | 60.4 | 67.1 |
a
—, mutation was not found.