FIG 7.

Macaque 13316 displayed an exceptional profile of control with no detectable increase in plasma viral loads but transient seroconversion after CD8⁺ cell depletion. (A) Frequencies of CD69⁺ EM CD4⁺ T cells from SIV controllers during the CD8⁺ depletion experiment. (B) Western blot SIV reactivity of plasma collected at various time points from macaque 13316, including pre- and postdepletion time points. (C) (Top) Summary of experiments involving the reactivation of viral replication from purified CD4⁺ T cells of macaque 13316. Samples were obtained from blood, LN, and purified CD4⁺ T cells. ++, time points at which SIV was isolated upon ConA activation and culture with IL-2; +, time points at which adjunction of heterologous PBMCs was needed for detectable SIV production; −, time points at which SIV could not be detected. (Bottom left) Two representative examples of SIV reactivation upon stimulation of CD4⁺ T cells isolated from blood or LN from 13316. (Bottom right) Spearman correlation between p27 concentration and reverse transcriptase (RT) activity in various culture supernatants (different time points in the summary) from macaque 13316 (●) and another CyM (○).