FIG 5.
Infection of human pDCs by MERS-CoV. (A) Quantification of viral N RNA in human or murine pDCs by qRT-PCR in the presence or absence of chloroquine, normalized to cellular GAPDH mRNA [ΔCT = CT(MERS vRNA) − CT(GAPDH mRNA)] at indicated time points after inoculation. (B to D) Immunoblot analysis of N protein expression in murine pDCs (B) or human pDCs (C and D) after inoculation with MERS-CoV (MOI of 3). pDCs of three different donors (D1 to D3 and D4 to D6) were infected in the presence of blocking anti-DPP4 serum (DPP4), the receptor binding domain of MERS-CoV S protein (RBD), or respective controls (Ctrl) or with UV-inactivated (UV) or live MERS-CoV in the presence (Chl) or absence (sham) of chloroquine. Cells were lysed at the indicated time points and subjected to analyses.