TABLE 7.

Detection of H5N1 HPAI virus shedding from oropharyngeal cavity at 2 DPC in chickens vaccinated with four different vaccine seed strains and challenged with one of three H5N1 HPAI viruses from Indonesia

Vaccine seed strain	Vaccine code	SMI-HAMD/06		Papua/06		PWT/06
		MDTa	Oral shedding at 2 DPCb	MDT	Oral Shedding at 2 DPC	MDT	Oral shedding at 2 DPC
PWT/06	P		1/10A (4.0A)	5.0c	2/10 (4.7)	NDd	ND
rgPWT/06	Q	ND	ND	ND	ND	2.0e	3/10A (3.5)A
rFPV-AI-H5–Legok/03	V		0/10A (≤3.9A)		0/10 (≤4.7)	9.5c	1/10A (4.2)B
Sham	U	2.0	10/10B (7.1B)	1.6	10/10 (7.1)	1.7	10/10B (6.3)C

^aMDT, mean time to death (in days).

^bData represent the number of virus-positive chickens/total number of chickens (log₁₀ mean virus titer). For number of virus-positive chickens/total number of chickens, different uppercase letters indicate statistically significant differences (Fisher's exact test, P < 0.05). For oral shedding, there were statistically significant differences in virus titers within each column (analysis of variance, P < 0.05), and different uppercase letters indicate statistically significant differences for log₁₀ mean virus titers between each vaccine seed strain (Student's t test, P < 0.05). For testing of statistical significance, samples in which virus was not detected were given a titer of the lowest detection limit minus 0.1 log₁₀.

^cTwo birds died, so the numbers were inadequate for statistical analysis.

^dND, not done.

^eOne bird died, so the numbers were inadequate for statistical analysis.