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. 2015 Jan 21;89(7):3804–3818. doi: 10.1128/JVI.02959-14

Fig 7.

Fig 7

Sendai virus induces formation of a novel apoptosis complex (Tom70/Hsp90/IRF3/Bax). (A and B) (Left) HEK293T cells were transfected with the indicated plasmids for 36 h and then subjected to immunoprecipitation with anti-HA antibody. (Right) HEK293 cells were stimulated with SeV for 24 h, and then cell lysates were subjected to immunoprecipitation with anti-IRF3 antibody or normal IgG. The immunoprecipitates were analyzed by immunoblotting with the indicated antibodies. (C) (Left) HEK293T cells were transfected with the indicated plasmids for 36 h and then subjected to immunoprecipitation with anti-MYC antibody. (Right) HEK293 cells were stimulated with SeV for 24 h, and then cell lysates were subjected to immunoprecipitation with anti-Tom70 antibody or normal IgG. The immunoprecipitates were analyzed by immunoblotting with the indicated antibodies. (D) HEK293 cells were challenged by SeV infection for the indicated times, and then lysates of whole cells (W) and of mitochondria isolated from the virus-infected cells (Mit) were immunoblotted with the indicated antibodies. (E) HEK293 cells were transfected with NC or Tom70 siRNA and then challenged with SeV for the indicated times. Lysates of whole cells (W) and of mitochondria (Mit) were resolved by SDS-PAGE and then immunoblotted with the indicated antibodies. (F) irf3−/− cells were infected with SeV for the indicated times. Lysates of whole cells (W) and of mitochondria (Mit) were resolved by SDS-PAGE and then immunoblotted with the indicated antibodies. (G) HEK293 cells were transfected with NC siRNA, Tom70 siRNA (upper panel), or Tom20 siRNA (lower panel) and then stimulated with SeV for 24 h, and then cell lysates were subjected to immunoprecipitation with anti-Bcl-2 antibody or normal IgG. (H) MEF cells were transfected with NC or Tom70 siRNA and then infected with SeV. Cells were stained with anti-cytochrome c antibody and then imaged by confocal microscopy. NC, negative control; W, whole-cell lysates; Mit, lysates of mitochondrial fraction; S, cytosolic part.