TABLE 1.
Primers used to generate pore mutations
| Mutation | Orientation | Primer sequence (5′–3′) |
|---|---|---|
| P223V | F | GGAGAAAATGTTGUACCAGTTCTTC |
| R | TCCTGTTAGTGTCCCAAAATATCTTG | |
| P223I | F | GGAGAAAATGTTATCCCAGTTCTTC |
| R | TCCTGTTAGTGTCCCAAAATATCTTG | |
| P223L | F | GGAGAAAATGTTCTACCAGTTCTTC |
| R | TCCTGTTAGTGTCCCAAAATATCTTG | |
| P223M | F | GGAGAAAATGTTATGCCAGTTCTTC |
| R | TCCTGTTAGTGTCCCAAAATATCTTG | |
| N221Q | F | GGAGGAGAACAAGTTCCTCCAG |
| R | TGTTAGTGTCCCAAAATATCTTGTG | |
| N221Y | F | GGAGGAGAATACGTTCCTCCAG |
| R | TGTTAGTGTCCCAAAATATCTTGTG | |
| N221W | F | GGAGGAGAATGGGTTCCTCCAG |
| R | TGTTAGTGTCCCAAAATATCTTGTG | |
| Q137Y | F | GCCAGTGTACGGCACCAG |
| R | TTCCCTGCACCATTGTCATGAG | |
| 137W | F | GCCAGTGTGGGGCACCAG |
| R | TTCCCTGCACCATTGTCATGAG |