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. 2015 Jan 28;89(7):3988–4001. doi: 10.1128/JVI.03584-14

FIG 1.

FIG 1

Recombinant DNA constructs. Depicted at the top of the figure are the wild-type (WT) NL4-3 strain of HIV-1 and its WT PrGag translation product. MA-BirA* and ΔMA-BirA* are variants of NL4-3 in which the open reading frame (ORF) for the promiscuous biotin ligase (BirA*) has been inserted into WT (MA-BirA*) or MA-deleted (ΔMA-BirA*) version of NL4-3. In MA-BirA*, the BirA* ORF has been inserted 12 codons upstream from the NL4-3 MA-CA cleavage junction, and the primary translation product is predicted to be a myristoylated PrGag protein of approximately 93 kDa. In ΔMA-BirA*, the BirA* ORF is also 12 codons upstream from the MA-CA cleavage junction and begins after codon 18 of MA, deleting MA residues 19 to 120; the primary translation product is predicted to be a myristoylated PrGag protein of approximately 82 kDa. LTR, long terminal repeat.